Molecular cloning of a cDNA encoding the feline CD62L

J Vet Med Sci. 2007 Jan;69(1):81-4. doi: 10.1292/jvms.69.81.

Abstract

We cloned a cDNA fragment encoding a feline homologue of L-selectin (CD62L). The extracellular region of the feline CD62L fragment contained a calcium-dependent (C-type) lectin domain, an epidermal growth factor-like domain, and two Sushi/CCP/SCR domains. The flow cytometric analysis confirmed that the feline CD62L molecule, which was expressed 293T cells, retained an epitope recognized by an anti-human CD62L monoclonal antibody (Leu-8).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / metabolism
  • Base Sequence
  • Cats / genetics*
  • Cell Line
  • Cloning, Molecular
  • DNA Primers
  • DNA, Complementary / genetics
  • Flow Cytometry
  • Humans
  • L-Selectin / genetics*
  • L-Selectin / metabolism*
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Sequence Analysis, DNA

Substances

  • Antibodies, Monoclonal
  • DNA Primers
  • DNA, Complementary
  • L-Selectin