Intragastric immunization with recombinant Lactobacillus casei expressing flagellar antigen confers antibody-independent protective immunity against Salmonella enterica serovar Enteritidis

Vaccine. 2007 May 4;25(18):3599-605. doi: 10.1016/j.vaccine.2007.01.055. Epub 2007 Jan 22.


A recombinant Lactobacillus casei expressing a flagellar antigen from Salmonella enterica serovar Enteritidis was constructed and evaluated as a mucosal vaccine. Intragastric immunization of the recombinant strain conferred protective immunity against Salmonella infection in mice. This immunization did not result in antigen-specific antibody in either feces or sera but induced the release of IFN-gamma on restimulation of primed lymphocytes ex vivo. The results suggested that the protective efficacy provided by flagellin-expressing L. casei is mainly attributable to cell-mediated immune responses. In addition, an adjuvant-type effect of the antigen delivery system with L. casei was also observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Female
  • Flagellin / genetics
  • Flagellin / immunology*
  • Flagellin / metabolism
  • Humans
  • Immunization / methods
  • Interferon-gamma / biosynthesis
  • Lactobacillus casei / genetics*
  • Lymphocyte Activation / immunology*
  • Mice
  • Mice, Inbred C3H
  • Recombination, Genetic
  • Salmonella Infections, Animal / microbiology
  • Salmonella Infections, Animal / prevention & control*
  • Salmonella Vaccines / administration & dosage*
  • Salmonella Vaccines / immunology
  • Salmonella enteritidis / genetics
  • Salmonella enteritidis / immunology*
  • Salmonella enteritidis / metabolism


  • Salmonella Vaccines
  • Flagellin
  • Interferon-gamma