MudrA-like sequences from rice and sugarcane cluster as two bona fide transposon clades and two domesticated transposases

Gene. 2007 May 1;392(1-2):117-25. doi: 10.1016/j.gene.2006.11.017. Epub 2006 Dec 6.

Abstract

The Mutator system of maize has been described as the most active and mutagenic plant transposon. The autonomous element MuDR contains two genes: mudrA encoding the transposase and mudrB whose product function remains undetermined. MudrA-like coding domain showed to be the most abundantly expressed transposon-related sequence in sugarcane transcriptome. A previous report identified the existence of at least four clades of mudrA-like sequences in sugarcane, rice and arabidopsis, which already existed prior to the Monocot-Eudicot split. To gain understanding about the abundance, distribution, copy number and diversity of mudrA-like sequences, a comparative study between sugarcane and rice was performed. As a result, it was possible to identify that copy number greatly differs and, at least in grasses, there was a class-specific amplification with a burst of Class II elements. Structural analyses performed on rice genomic sequences revealed that while Class I and Class II clades comprise elements with transposon features, Class III and Class IV no longer possess TIRs and correspond to domesticated transposases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chromosome Mapping
  • Chromosomes, Plant
  • Conserved Sequence
  • DNA Transposable Elements*
  • Gene Dosage
  • Genes, Plant
  • Oryza / genetics*
  • Phylogeny
  • Saccharum / genetics*
  • Transposases / genetics*

Substances

  • DNA Transposable Elements
  • Transposases