Production of aminoglycosides in non-aminoglycoside producing Streptomyces lividans TK24

Bimala Subba; Nagendra Prasad Kurumbang; Young Soo Jung; Yeo Joon Yoon; Hei Chan Lee; Kwangkyoung Liou; Jae Kyung Sohng

doi:10.1016/j.bmcl.2007.01.035

Production of aminoglycosides in non-aminoglycoside producing Streptomyces lividans TK24

Bioorg Med Chem Lett. 2007 Apr 1;17(7):1892-6. doi: 10.1016/j.bmcl.2007.01.035. Epub 2007 Jan 19.

Authors

Bimala Subba¹, Nagendra Prasad Kurumbang, Young Soo Jung, Yeo Joon Yoon, Hei Chan Lee, Kwangkyoung Liou, Jae Kyung Sohng

Affiliation

¹ Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam 336-708, Republic of Korea.

PMID: 17289380
DOI: 10.1016/j.bmcl.2007.01.035

Abstract

The pRBM4 cosmid, which harbors a putative cluster of genes spanning a 31.8-kb chromosomal region of the ribostamycin producer Streptomyces ribosidificus ATCC 21294, was heterologously expressed in Streptomyces lividans TK24. ESI-MS/MS, HPLC, and LC-ESI MS analyses showed that the transformation gave rise to ribostamycin production in various culture broths. This is the first report of heterologous aminoglycoside production.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aminoglycosides / chemistry*
Anti-Bacterial Agents / chemical synthesis*
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology
Biochemistry / methods
Chemistry, Pharmaceutical / methods*
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Cosmids
Drug Design
Models, Chemical
Molecular Conformation
Plasmids / metabolism
Ribostamycin / chemistry*
Spectrometry, Mass, Electrospray Ionization
Streptomyces lividans / metabolism*

Substances

Aminoglycosides
Anti-Bacterial Agents
Ribostamycin