Induction of HIV-specific memory T-cell responses by topical DermaVir vaccine

Vaccine. 2007 Apr 20;25(16):3070-4. doi: 10.1016/j.vaccine.2007.01.024. Epub 2007 Jan 22.

Abstract

In vivo antigen expression by plasmid DNA could provide a potent and cost-effective vaccine platform if its immunogenicity were improved to induce antigen-specific memory T-cell responses. To study these immune responses, we compared naked DNA vaccine with topical DermaVir formulated with the same HIV-1 (Gag) DNA in the mouse model. Topical DermaVir induced HIV-specific effector memory CD8(+) T-cell responses, which were 2.4-fold higher than those elicited by intramuscular injection of naked DNA. DermaVir, but not naked DNA vaccination, induced robust HIV-specific central memory T-cells responses, which are likely to be more efficient in mediating protective immunity. DermaVir formulation combined with topical administration provides an improved immunogenicity of antigen-expressing DNA vaccines.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • AIDS Vaccines / administration & dosage*
  • AIDS Vaccines / genetics
  • AIDS Vaccines / immunology*
  • Acquired Immunodeficiency Syndrome / immunology
  • Acquired Immunodeficiency Syndrome / prevention & control*
  • Administration, Topical
  • Animals
  • HIV Antibodies / blood
  • HIV-1 / genetics
  • HIV-1 / immunology*
  • Immunologic Memory
  • Mice
  • Mice, Inbred BALB C
  • Plasmids
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology*
  • Vaccination / methods

Substances

  • AIDS Vaccines
  • DermaVir
  • HIV Antibodies