The cytosolic ribonuclease inhibitor contributes to intracellular redox homeostasis

FEBS Lett. 2007 Mar 6;581(5):930-4. doi: 10.1016/j.febslet.2007.01.072. Epub 2007 Feb 6.

Abstract

The hypothesis that the cytosolic RNase inhibitor (cRI) has a role in the protection of the cellular redox homeostasis was investigated testing the effects of oxidants and anti-oxidants on normal, primary endothelial HUVE cells, and malignant HeLa cells, before and after their engineering into cRI-deprived cells. We found that cRI plays an important, possibly a key, physiological role in the protection of cells from redox stress, as demonstrated by decreased GSH levels as well as increased oxidant-induced DNA damage in cRI deprived cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / metabolism
  • Carrier Proteins / antagonists & inhibitors
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cells, Cultured
  • Endothelial Cells / drug effects
  • Endothelial Cells / metabolism
  • Enzyme Inhibitors / metabolism*
  • Glutathione / metabolism
  • HeLa Cells
  • Homeostasis
  • Humans
  • Oxidation-Reduction
  • RNA Interference
  • RNA, Small Interfering / genetics
  • Ribonucleases / antagonists & inhibitors*

Substances

  • Antioxidants
  • Carrier Proteins
  • Enzyme Inhibitors
  • RNA, Small Interfering
  • RNH1 protein, human
  • Ribonucleases
  • Glutathione