Apoptosis repressor with caspase recruitment domain (ARC) inhibits myogenic differentiation

FEBS Lett. 2007 Mar 6;581(5):879-84. doi: 10.1016/j.febslet.2007.01.050. Epub 2007 Feb 2.


Apoptosis repressor with caspase recruitment domain (ARC), an anti-apoptotic protein, is highly expressed in differentiated heart and skeletal muscle. Apoptosis and differentiation share numerous common pathways; therefore, we examined the impact of ARC on H9c2-myoblast differentiation. We demonstrate that ARC expression levels increase and stabilize upon differentiation. ARC-overexpression in pre-differentiated H9c2-cells suppresses differentiation; indicated by increased myotube formation, nuclear fusion and expression of the differentiation markers myogenin and troponin-T. ARC-overexpression inhibited myoblast differentiation associated caspase-3 activation, suggesting ARC inhibits myogenic differentiation through caspase inhibition. In summary, we show a novel role for ARC in the regulation of muscle differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis Regulatory Proteins / genetics
  • Apoptosis Regulatory Proteins / metabolism*
  • Caspase 3 / metabolism
  • Cell Differentiation / physiology
  • Cell Line
  • Enzyme Activation
  • Gene Expression
  • Muscle Development / physiology
  • Muscle Proteins / genetics
  • Muscle Proteins / metabolism*
  • Myoblasts, Cardiac / cytology*
  • Myoblasts, Cardiac / metabolism*
  • Rats
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Transfection


  • Apoptosis Regulatory Proteins
  • Muscle Proteins
  • Nol3 protein, rat
  • Recombinant Proteins
  • Casp3 protein, rat
  • Caspase 3