Affinity purification of human tau proteins and the construction of a sensitive sandwich enzyme-linked immunosorbent assay for human tau detection

J Neurochem. 1992 Feb;58(2):548-53. doi: 10.1111/j.1471-4159.1992.tb09754.x.


Immunoaffinity chromatography with a monoclonal antibody produced against bovine tau protein was used to purify tau proteins from human brain. Fifty grams of brain tissue yielded approximately 2 mg of pure tau proteins. The affinity-purified human tau was used to produce a high-titered rabbit anti-human tau serum. The monoclonal anti-tau antibody and the polyclonal rabbit anti-tau serum were then used to construct a sandwich enzyme-linked immunosorbent assay for detection of human tau proteins, with a sensitivity of 1 ng/ml.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Chromatography, Affinity*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Humans
  • Immune Sera
  • tau Proteins / isolation & purification*


  • Antibodies, Monoclonal
  • Immune Sera
  • tau Proteins