Inhibition of gastric H+, K+-ATPase and Helicobacter pylori growth by phenolic antioxidants of Zingiber officinale

Mol Nutr Food Res. 2007 Mar;51(3):324-32. doi: 10.1002/mnfr.200600202.

Abstract

Ulcer is a common global problem characterized by acute gastric irritability, bleeding, etc. due to either increased gastric cell proton potassium ATPase activity (PPA) or perturbation of mucosal defence. Helicobacter pylori has been identified as a major ulcerogen in addition to oxidative stress and nonsteroidal anti-inflammatory drugs. In this paper, we report ginger-free phenolic (GRFP) and ginger hydrolysed phenolic (GRHP) fractions of ginger (Zingiber officinale) as potent inhibitors of PPA and H. pylori growth. GRFP and GRHP inhibited PPA at an IC(50) of 2.9 +/- 0.18 and 1.5 +/- 0.12 microg/mL, exhibiting six- to eight-fold better potency over lansoprazole. GRFP is constituted by syringic (38%), gallic (18%) and cinnamic (14%) acids and GRHP by cinnamic (48%), p-coumaric (34%) and caffeic (6%) acids as major phenolic acids. GRFP and GRHP further exhibited free radical scavenging (IC(50) 1.7 +/- 0.07 and 2.5 +/- 0.16), inhibition of lipid peroxidation (IC(50) 3.6 +/- 0.21 and 5.2 +/- 0.46), DNA protection (80% at 4 microg) and reducing power abilities (80-338 U/g) indicating strong antioxidative properties. GRFP and GRHP may thus be potential in-expensive multistep blockers against ulcer.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Pyridinylmethylsulfinylbenzimidazoles / pharmacology
  • Antioxidants / pharmacology
  • Caffeic Acids / pharmacology
  • Cinnamates / pharmacology
  • Coumaric Acids / pharmacology
  • Enzyme Inhibitors / pharmacology
  • Gallic Acid / analogs & derivatives
  • Gallic Acid / pharmacology
  • Gastric Mucosa / enzymology*
  • Ginger / chemistry*
  • Helicobacter pylori / drug effects*
  • Helicobacter pylori / growth & development*
  • Lansoprazole
  • Lipid Peroxidation / drug effects
  • Phenols / pharmacology*
  • Propionates
  • Proton Pump Inhibitors*

Substances

  • 2-Pyridinylmethylsulfinylbenzimidazoles
  • Antioxidants
  • Caffeic Acids
  • Cinnamates
  • Coumaric Acids
  • Enzyme Inhibitors
  • Phenols
  • Propionates
  • Proton Pump Inhibitors
  • Lansoprazole
  • Gallic Acid
  • syringic acid
  • trans-3-(4'-hydroxyphenyl)-2-propenoic acid
  • cinnamic acid
  • caffeic acid