Differentiation of wine lactic acid bacteria species based on RFLP analysis of a partial sequence of rpoB gene

Olivier Claisse; Vincent Renouf; Aline Lonvaud-Funel

doi:10.1016/j.mimet.2007.01.004

Differentiation of wine lactic acid bacteria species based on RFLP analysis of a partial sequence of rpoB gene

J Microbiol Methods. 2007 May;69(2):387-90. doi: 10.1016/j.mimet.2007.01.004. Epub 2007 Jan 19.

Authors

Olivier Claisse¹, Vincent Renouf, Aline Lonvaud-Funel

Affiliation

¹ UMR oenologie, ISVV INRA-Université Victor Segalen Bordeaux2, 351 cours de la libération, 33405 Talence cedex, France.

PMID: 17303271
DOI: 10.1016/j.mimet.2007.01.004

Abstract

PCR-RFLP analysis of rpoB sequences were used to identify lactic acid bacteria (LAB) species commonly isolated from wine. Strains of seven cocci and 12 lactobacilli species could be identified after single digestion with AciI for the cocci and two or three digestions (AciI, HinfI and MseI) for the rods and preceded by colonies isolation on solid selective medium and microscope observation to distinguish cocci and rods cells.

MeSH terms

DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA-Directed RNA Polymerases / chemistry
DNA-Directed RNA Polymerases / genetics
Deoxyribonucleases, Type II Site-Specific / metabolism
Genes, Bacterial*
Lactobacillaceae / classification
Lactobacillaceae / genetics*
Phylogeny
Polymerase Chain Reaction / methods
Polymorphism, Restriction Fragment Length*
Wine / microbiology*

Substances

DNA, Bacterial
DNA-Directed RNA Polymerases
RNA polymerase beta subunit
endodeoxyribonuclease AciI
endodeoxyribonuclease MseI
Deoxyribonucleases, Type II Site-Specific
GANTC-specific type II deoxyribonucleases