Almost forgotten or latest practice? AFLP applications, analyses and advances

Trends Plant Sci. 2007 Mar;12(3):106-17. doi: 10.1016/j.tplants.2007.02.001. Epub 2007 Feb 14.


Amplified fragment length polymorphism (AFLP) DNA fingerprinting is a firmly established molecular marker technique, with broad applications in population genetics, shallow phylogenetics, linkage mapping, parentage analyses, and single-locus PCR marker development. Technical advances have presented new opportunities for data analysis, and recent studies have addressed specific areas of the AFLP technique, including comparison to other genotyping methods, assessment of errors, homoplasy, phylogenetic signal and appropriate analysis techniques. Here we provide a synthesis of these areas and explore new directions for the AFLP technique in the genomic era, with the aim of providing a review that will be applicable to all AFLP-based studies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Chromosome Mapping
  • DNA Fingerprinting*
  • Genotype
  • Phylogeny
  • Plants / genetics*
  • Polymerase Chain Reaction*
  • Polymorphism, Restriction Fragment Length*