Abstract
In this study, we isolated and analyzed a small heat shock protein (HSP) of Ostertagia ostertagi (Oo-HSP18). Oo-hsp18 is encoded by a single-copy gene and the full-length cDNA represents an 18-kDa protein. The expression of Oo-hsp18 is highly stage specific and restricted to the adult stage. The protein is synthesized in a tissue-specific manner and localized in the body muscle layer. The levels of Oo-hsp18 mRNAs are sharply induced by heat shock but not by other stressors such as levamisole and H2O2. A vaccination trial with recombinant Oo-HSP18 failed to protect calves against a challenge infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Blotting, Western
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Cattle
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Cattle Diseases / prevention & control
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Cells, Cultured
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / isolation & purification
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DNA, Helminth / chemistry
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DNA, Helminth / isolation & purification
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Gene Expression
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Heat-Shock Proteins, Small / biosynthesis
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Heat-Shock Proteins, Small / chemistry
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Heat-Shock Proteins, Small / genetics
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Heat-Shock Proteins, Small / immunology*
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Hot Temperature
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Male
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Molecular Sequence Data
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Ostertagia / genetics
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Ostertagia / immunology*
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Ostertagiasis / prevention & control
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Ostertagiasis / veterinary
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Polymerase Chain Reaction
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Sequence Alignment
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Sequence Homology, Amino Acid
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Spodoptera
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Transcription, Genetic
Substances
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DNA, Complementary
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DNA, Helminth
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Heat-Shock Proteins, Small
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Recombinant Proteins