A small heat shock protein of Ostertagia ostertagi: stage-specific expression, heat inducibility, and protection trial

J Parasitol. 2006 Dec;92(6):1244-50. doi: 10.1645/GE-871R.1.

Abstract

In this study, we isolated and analyzed a small heat shock protein (HSP) of Ostertagia ostertagi (Oo-HSP18). Oo-hsp18 is encoded by a single-copy gene and the full-length cDNA represents an 18-kDa protein. The expression of Oo-hsp18 is highly stage specific and restricted to the adult stage. The protein is synthesized in a tissue-specific manner and localized in the body muscle layer. The levels of Oo-hsp18 mRNAs are sharply induced by heat shock but not by other stressors such as levamisole and H2O2. A vaccination trial with recombinant Oo-HSP18 failed to protect calves against a challenge infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Western
  • Cattle
  • Cattle Diseases / prevention & control
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification
  • DNA, Helminth / chemistry
  • DNA, Helminth / isolation & purification
  • Gene Expression
  • Heat-Shock Proteins, Small / biosynthesis
  • Heat-Shock Proteins, Small / chemistry
  • Heat-Shock Proteins, Small / genetics
  • Heat-Shock Proteins, Small / immunology*
  • Hot Temperature
  • Male
  • Molecular Sequence Data
  • Ostertagia / genetics
  • Ostertagia / immunology*
  • Ostertagiasis / prevention & control
  • Ostertagiasis / veterinary
  • Polymerase Chain Reaction
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Spodoptera
  • Transcription, Genetic

Substances

  • DNA, Complementary
  • DNA, Helminth
  • Heat-Shock Proteins, Small
  • Recombinant Proteins