Identification and characterization of microRNAs from the bovine adipose tissue and mammary gland

FEBS Lett. 2007 Mar 6;581(5):981-8. doi: 10.1016/j.febslet.2007.01.081. Epub 2007 Feb 7.


We report the identification of bovine miRNAs by cloning small RNAs from adipose tissue and the mammary gland. Fifty-nine distinct miRNAs were identified, five of them were not homologous to known mammalian miRNAs, and many of them had 3' and/or 5' end variants. Ribonuclease protection assays indicated that miR-23a and miR-24, whose genes are closely located on the same chromosome, were co-expressed in different tissues. The assays also suggested a role for several miRNAs in the mammary gland and a role for miR-133, a previously known skeletal and cardiac muscle-specific miRNA, in the rumen, an organ unique to the ruminant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / metabolism*
  • Animals
  • Base Sequence
  • Cattle
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Female
  • Gene Expression
  • Mammary Glands, Animal / metabolism*
  • MicroRNAs / genetics*
  • MicroRNAs / isolation & purification
  • Multigene Family
  • Rumen / metabolism
  • Tissue Distribution


  • DNA, Complementary
  • MicroRNAs