Schistosoma mansoni antigen-driven interleukin-10 production in infected asthmatic individuals

Mem Inst Oswaldo Cruz. 2006 Sep;101 Suppl 1:339-43. doi: 10.1590/s0074-02762006000900055.

Abstract

Asthmatics infected with Schistosoma mansoni have a less severe course of asthma and an inhibition of the Th2 inflammatory response that seems to be mediated by interleukin (IL-10). The objective of this study was to evaluate the capacity of some S. mansoni antigens to stimulate IL-10 production in vitro by cells of asthmatic infected individuals. Peripheral bloods mononuclear cells were stimulated with the S. mansoni recombinant antigens Sm22.6, Sm14, P24, and PIII antigen. IL-10 was measured in the supernatants of cultures. As the recombinant antigens were cloned in Escherichia coli, we blocked contaminant endotoxin with polymyxin B added to the cultures. We demonstrated that all antigens used drove high production of IL-10 in S. mansoni infected individuals (n = 13, 408 +/- 514 and 401 +/- 383 pg/ml, 484 +/- 245 pg/ml, 579 +/- 468 pg/ml, respectively). In asthmatics infected with S. mansoni (n = 21) rP24 induced higher levels of IL-10 (565 +/- 377 pg/ml) when compared to PIII, rSm14 and rSm22.6 (184 +/- 209 pg/ml; 292 +/- 243 pg/ml; 156 +/- 247 pg/ml, respectively).

Conclusion: the S. mansoni antigens evaluated in this study stimulated IL-10 production by cells from infected individuals and therefore they have the potential to be used as a modulator of the inflammatory response in asthma.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Antigens, Helminth / immunology*
  • Asthma / complications
  • Asthma / immunology*
  • Asthma / parasitology
  • Cells, Cultured
  • Child
  • Female
  • Humans
  • Interleukin-10 / biosynthesis*
  • Leukocytes, Mononuclear / immunology
  • Leukocytes, Mononuclear / parasitology
  • Male
  • Polymyxin B / pharmacology
  • Recombinant Proteins / immunology*
  • Schistosomiasis mansoni / complications
  • Schistosomiasis mansoni / immunology*

Substances

  • Antigens, Helminth
  • Recombinant Proteins
  • Interleukin-10
  • Polymyxin B