A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 11q: comparison with chromosome 21q

DNA Seq. 2006 Aug;17(4):300-6. doi: 10.1080/10425170600886128.


It was generally believed that autosomal CpG islands (CGIs) escape methylation. However, our comprehensive analysis of allelic methylation status of 149 CGIs on human chromosome 21q revealed that a sizable fraction of them are methylated on both alleles even in normal blood cells. Here, we performed a similar analysis of 656 CGIs on chromosome 11q, which is gene-rich in contrast with 21q. The results indicate that 11q contains less methylated CGIs, especially those with tandem repeats and those in the coding or 3'-untranslated regions (UTRs), than 21q. Thus, methylation status of CGIs may substantially differ from one chromosome to another.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosomes, Human, Pair 11 / genetics*
  • Chromosomes, Human, Pair 21 / genetics*
  • CpG Islands / genetics*
  • DNA Methylation*
  • DNA Primers
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA


  • DNA Primers