Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors

Jpn J Infect Dis. 2007 Feb;60(1):45-7.


We constructed a pair of Nocardia-Escherichia coli shuttle vectors, pNV18 and pNV19, by combining the mycobacterial plasmid pAL5000 with the E. coli vector pK18 or pK19. These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • Drug Resistance, Bacterial
  • Escherichia coli / genetics*
  • Genetic Vectors / genetics*
  • Lac Operon
  • Mycobacterium / genetics
  • Nocardia / genetics*
  • Plasmids / genetics
  • Tobramycin / pharmacology
  • Transformation, Bacterial / genetics


  • Tobramycin