Vaccination of horses against strangles using recombinant antigens from Streptococcus equi

Vaccine. 2007 May 4;25(18):3629-35. doi: 10.1016/j.vaccine.2007.01.060. Epub 2007 Jan 22.


Strangles is an upper respiratory tract infection in horses, which is highly contagious and one of the more costly diseases of the horse. Three recombinant antigens were used to vaccinate horses, which were then experimentally challenged with Streptococcus equi, the causative agent for strangles. The vaccinated horses showed significantly reduced bacterial growth (p=0.02) and nasal discharge (p=0.0004), a typical symptom of strangles. Other clinical signs of strangles were also reduced and at post mortem examination, lower rate of empyaema or scarring of the guttural pouches was found in the vaccinated group (p=0.01). The antigens used were EAG (alpha2-macroglobulin, albumin, and IgG-binding protein), CNE (a collagen-binding protein), and SclC (a collagen-like protein). The adjuvant used was Abisco, a saponin derived matrix. No adverse effects were observed following vaccination with the antigens and adjuvant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Horse Diseases / microbiology
  • Horse Diseases / prevention & control*
  • Horses
  • Immunoglobulin G / blood
  • Membrane Proteins / genetics
  • Membrane Proteins / immunology
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology*
  • Respiratory Tract Infections / microbiology
  • Respiratory Tract Infections / prevention & control
  • Respiratory Tract Infections / veterinary*
  • Streptococcal Infections / microbiology
  • Streptococcal Infections / prevention & control
  • Streptococcal Infections / veterinary
  • Streptococcal Vaccines / administration & dosage
  • Streptococcal Vaccines / genetics
  • Streptococcal Vaccines / immunology*
  • Streptococcus equi / genetics
  • Streptococcus equi / immunology*
  • Streptococcus equi / pathogenicity
  • Vaccination / veterinary


  • Antigens, Bacterial
  • Immunoglobulin G
  • Membrane Proteins
  • Recombinant Proteins
  • Streptococcal Vaccines