Biosynthesis of vitamin B12 in Pseudomonas denitrificans: the biosynthetic sequence from precorrin-6y to precorrin-8x is catalyzed by the cobL gene product

F Blanche; A Famechon; D Thibaut; L Debussche; B Cameron; J Crouzet

doi:10.1128/jb.174.3.1050-1052.1992

Biosynthesis of vitamin B12 in Pseudomonas denitrificans: the biosynthetic sequence from precorrin-6y to precorrin-8x is catalyzed by the cobL gene product

J Bacteriol. 1992 Feb;174(3):1050-2. doi: 10.1128/jb.174.3.1050-1052.1992.

Authors

F Blanche¹, A Famechon, D Thibaut, L Debussche, B Cameron, J Crouzet

Affiliation

¹ Département Analyse, Centre de Recherche de Vitry-Alfortville, Vitry-sur-Seine, France.

Abstract

A protein catalyzing methylation at C-5 and C-15 and decarboxylation of the acetic acid side chain at C-12 on precorrin-6y to yield precorrin-8x was purified to homogeneity from a recombinant strain of Pseudomonas denitrificans. It was sequenced at the N terminus and shown to be encoded by the cobL gene.

MeSH terms

Amino Acid Sequence
Bacterial Proteins / genetics*
Decarboxylation
Genes, Bacterial
Methylation
Molecular Sequence Data
Pseudomonas / enzymology*
Uroporphyrins / metabolism*
Vitamin B 12 / biosynthesis*

Substances

Bacterial Proteins
Uroporphyrins
precorrin 6y
precorrin 8x
Vitamin B 12