Biosynthesis of vitamin B12 in Pseudomonas denitrificans: the biosynthetic sequence from precorrin-6y to precorrin-8x is catalyzed by the cobL gene product

J Bacteriol. 1992 Feb;174(3):1050-2. doi: 10.1128/jb.174.3.1050-1052.1992.


A protein catalyzing methylation at C-5 and C-15 and decarboxylation of the acetic acid side chain at C-12 on precorrin-6y to yield precorrin-8x was purified to homogeneity from a recombinant strain of Pseudomonas denitrificans. It was sequenced at the N terminus and shown to be encoded by the cobL gene.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Decarboxylation
  • Genes, Bacterial
  • Methylation
  • Molecular Sequence Data
  • Pseudomonas / enzymology*
  • Uroporphyrins / metabolism*
  • Vitamin B 12 / biosynthesis*


  • Bacterial Proteins
  • Uroporphyrins
  • precorrin 6y
  • precorrin 8x
  • Vitamin B 12