Imaging receptor microdomains on leukocyte subsets in live mice

Nat Methods. 2007 Mar;4(3):219-22. doi: 10.1038/nmeth1018. Epub 2007 Feb 25.

Abstract

We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45+ F4/80- Gr-1+ neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45+ B220+ B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1+ leukocytes are not firmly stationary but actively migrate on TNF-alpha-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Surface / immunology*
  • Fluorescence Polarization Immunoassay / methods*
  • Leukocytes / cytology*
  • Leukocytes / immunology*
  • Membrane Microdomains / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Fluorescence, Multiphoton / methods*

Substances

  • Antigens, Surface