DmOAZ, the unique Drosophila melanogaster OAZ homologue is involved in posterior spiracle development

Dev Genes Evol. 2007 Mar;217(3):197-208. doi: 10.1007/s00427-007-0134-7. Epub 2007 Feb 15.

Abstract

In this paper, we study DmOAZ, the unique Drosophila melanogaster homologue of the OAZ zinc finger protein family. We show partial conservation of the zinc finger organization between DmOAZ and the vertebrate members of this family. We determine the exon/intron structure of the dmOAZ gene and deduce its open reading frame. Reverse transcriptase-polymerase chain reaction analysis shows that dmOAZ is transcribed throughout life. In the embryo, strongest DmOAZ expression is observed in the posterior spiracles. We suggest that dmOAZ acts as a secondary target of the Abd-B gene in posterior spiracle development, downstream of cut and ems. In a newly created loss-of-function mutant, dmOAZ ( 93 ), the "filzkörper" part of the posterior spiracles, is indeed structurally abnormal. The dmOAZ ( 93 ) mutant is a larval lethal, a phenotype that may be linked to the spiracular defect. Given the dmOAZ ( 93 ) mutant as a new tool, the fruit fly may provide an alternative model for analyzing in vivo the functions of OAZ family members.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Drosophila Proteins / chemistry
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / embryology*
  • Drosophila melanogaster / metabolism*
  • Embryo, Nonmammalian / metabolism
  • Homeodomain Proteins / metabolism
  • Larva
  • Molecular Sequence Data
  • Morphogenesis
  • Mutation / genetics
  • Nuclear Proteins / metabolism
  • Phenotype
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Respiratory System / embryology*
  • Respiratory System / metabolism*
  • Sequence Analysis, Protein
  • Sequence Homology, Amino Acid*
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic

Substances

  • Drosophila Proteins
  • Homeodomain Proteins
  • Nuclear Proteins
  • OAZ protein, Drosophila
  • RNA, Messenger
  • Transcription Factors
  • ct protein, Drosophila
  • ems protein, Drosophila