A novel vitamin C preparation enhances neurite formation and fibroblast adhesion and reduces xenobiotic-induced T-cell hyperactivation

Med Sci Monit. 2007 Mar;13(3):BR51-8.


Background: Vitamin C (ascorbic acid, ascorbate) has been shown to enhance neurite outgrowth, promote fibroblast adhesion during wound healing, and reduce xenobiotic-induced leukocyte hyperactivity and inflammatory damage. In this study, a comparison was made between Ester-C and PureWay-C on these various cellular activities.

Material/methods: PC12 cells were stimulated to form neurites with nerve growth factor, NIH 3T3 fibroblasts were seeded on fibronectin and H9 T-cells were stimulated to aggregate with the pyrethroid pesticide bifenthrin. The rate of neurite formation, fibroblast adhesion and T-cell homotypic aggregation was then measured in the absence and presence of various formulations of vitamin C including Ester-C and PureWay-CTM.

Results: With PureWay-C treatment, 12% of PC12 cells extended neurites within one hour of treatment and 45% of the cells extended neurites by hour nine. With Ester-C, 0% and 15% extended neurites at one and nine hours, respectively. NIH-3T3 fibroblast adhesion to fibronectin was enhanced by 4.7-fold with a 30 minute PureWay-CTM treatment while Ester-C increased fibroblast adhesion by only 1.5 fold. Further, PureWay-CTM reduced pesticide-mediated T-cell homotypic aggregation by 83% within 30 minutes of treatment while the reduction seen with Ester-C was only 33%.

Conclusions: These data confirm the previous observations that vitamin C supplementation can promote neurite outgrowth, increase fibroblast adhesion and reduce xenobiotic induce immunocytes aggregation. More importantly, these data show that PureWay-C has a faster and greater beneficial effect on these parameters when compared to other vitamin C formulations.

MeSH terms

  • Animals
  • Ascorbic Acid / pharmacology*
  • Cell Adhesion / drug effects
  • Cell Aggregation / drug effects
  • Dose-Response Relationship, Drug
  • Fibroblasts / cytology*
  • Fibroblasts / drug effects*
  • Fibronectins / metabolism
  • Humans
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / immunology*
  • Mice
  • NIH 3T3 Cells
  • Nerve Growth Factors / pharmacology
  • Neurites / drug effects*
  • PC12 Cells
  • Phytohemagglutinins / pharmacology
  • Pyrethrins / pharmacology
  • Rats
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology*
  • Xenobiotics / pharmacology*


  • Fibronectins
  • Nerve Growth Factors
  • Phytohemagglutinins
  • Pyrethrins
  • Xenobiotics
  • bifenthrin
  • Ascorbic Acid