Pituitary adenylate cyclase activating polypeptide stimulates insulin release from the isolated perfused rat pancreas

Life Sci. 1992;50(4):257-61. doi: 10.1016/0024-3205(92)90332-j.


Pituitary adenylate cyclase activating polypeptide (PACAP) is a novel hypothalamic peptide structurally related to vasoactive intestinal peptide (VIP) and glucagon like peptide-1(7-36) amide (tGLP-1) in its N-terminal portion. Therefore, their levels of insulinotropic potency were compared using an isolated rat pancreas perfusion. It was found that 0.1 nM PACAP (1-27) amide (PACAP27) significantly stimulated insulin release under a perfusate glucose concentration of 5.5 mM, whereas 1 nM PACAP27 did not under a perfusate glucose concentration of 2.8 mM. The potency was evaluated as tGLP-1 greater than PACAP27 greater than VIP. These results indicate that PACAP is a glucagon superfamily peptide which stimulates insulin release in a glucose dependent manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Insulin / metabolism*
  • Insulin Secretion
  • Islets of Langerhans / metabolism*
  • Molecular Sequence Data
  • Neuropeptides / chemistry
  • Neuropeptides / pharmacology*
  • Perfusion
  • Pituitary Adenylate Cyclase-Activating Polypeptide
  • Rats


  • Adcyap1 protein, rat
  • Insulin
  • Neuropeptides
  • Pituitary Adenylate Cyclase-Activating Polypeptide