Copper inhibition of hydrogen peroxide-induced hypertrophy in embryonic rat cardiac H9c2 cells

Exp Biol Med (Maywood). 2007 Mar;232(3):385-9.

Abstract

Previous studies have shown that dietary copper deficiency causes cardiac hypertrophy and depression of vascular epithelial growth factor (VEGF) expression in mouse model. Copper replenishment in the diet reverses cardiac hypertrophy and restores VEGF expression. The present study was undertaken to specifically determine the role of VEGF in copper effect on cell hypertrophy. Embryonic rat cardiac H9c2 cells were exposed to hydrogen peroxide to develop hypertrophy, determined by increases in cell size and total protein content. Copper addition at 5 microM in cultures suppressed cell hypertrophy. In the presence of anti-VEGF antibody, copper inhibitory effect on cell hypertrophy was blunted, and VEGF alone mimicked the inhibitory effect of copper. The results thus demonstrated that VEGF is critically involved in copper inhibition of cell hypertrophy induced by hydrogen peroxide in the H9c2 cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • Cell Enlargement / drug effects*
  • Cell Line
  • Cell Nucleus / drug effects
  • Cell Nucleus / metabolism
  • Copper / pharmacology*
  • Copper Sulfate / pharmacology
  • Hydrogen Peroxide / toxicity*
  • Myoblasts / cytology
  • Myoblasts / drug effects*
  • Myoblasts / metabolism
  • Myocardium / cytology
  • Myocardium / metabolism
  • Rats
  • Vascular Endothelial Growth Factor A / immunology
  • Vascular Endothelial Growth Factor A / pharmacology

Substances

  • Antibodies, Monoclonal
  • Vascular Endothelial Growth Factor A
  • Copper
  • Hydrogen Peroxide
  • Copper Sulfate