Abnormality of CD4(+)CD25(+) regulatory T cells in idiopathic thrombocytopenic purpura

Eur J Haematol. 2007 Feb;78(2):139-43. doi: 10.1111/j.1600-0609.2006.00780.x.


Objectives: The aim of this study was to explore the profile and function of CD4(+)CD25(+) regulatory T cells (Treg cells) in idiopathic thrombocytopenic purpura (ITP) patients.

Methods: Treg cell numbers were analyzed by flow cytometric analysis in peripheral blood mononuclear cells collected from healthy donors or patients with ITP. Quantification of cell proliferation was assayed by an enzyme-linked immunosorbent assay kit, based on the measurement of BrdU incorporation during DNA synthesis.

Results: The percentage of Treg cells was significantly decreased in ITP patients in active and non-remission state(5.79 +/- 1.22%) when compared with the patients in remission(11.63 +/- 4.56%) and to healthy subjects(12.68 +/- 3.59%). The suppressive activity of Treg cells in ITP patients was also found to be impaired.

Conclusion: These results suggest that decreased number and function of Treg cells might be one of mechanisms that cause immune regulation dysfunction in ITP.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • CD4 Antigens / analysis
  • Cell Division
  • Cells, Cultured / immunology
  • DNA Replication
  • Female
  • Humans
  • Immunosuppressive Agents / therapeutic use
  • Interleukin-2 Receptor alpha Subunit / analysis
  • Lymphocyte Count
  • Male
  • Middle Aged
  • Prednisolone / therapeutic use
  • Purpura, Thrombocytopenic, Idiopathic / drug therapy
  • Purpura, Thrombocytopenic, Idiopathic / immunology*
  • Purpura, Thrombocytopenic, Idiopathic / pathology
  • Remission Induction
  • Spherocytosis, Hereditary / immunology
  • Spherocytosis, Hereditary / pathology
  • Spleen / immunology
  • Spleen / pathology
  • T-Lymphocytes, Regulatory / chemistry
  • T-Lymphocytes, Regulatory / immunology
  • T-Lymphocytes, Regulatory / pathology*


  • CD4 Antigens
  • Immunosuppressive Agents
  • Interleukin-2 Receptor alpha Subunit
  • Prednisolone