Auxin pulse treatment holds the potential to enhance efficiency and practicability of somatic embryogenesis in potato

Plant Cell Rep. 2007 Jul;26(7):945-50. doi: 10.1007/s00299-007-0319-6. Epub 2007 Feb 28.

Abstract

The objective of the current study was to simplify existing somatic embryogenesis systems in potato (Solanum tuberosum L.) cv. Desiree. The project targeted the agar-based induction phase of the potato somatic embryogenesis process as the key area for improvement. Experiments were established to ascertain the effect of a 2,4-D (2,4 dichlorophenoxyacetic acid) pulse, applied to the primary internodal section explant source and its subsequent effect on embryo induction. Parameters tested were the duration of the auxin pulse in a range from 0 to 300 min, and the concentrations of 2,4-D applied, in a range from 0 to 5,120 microM. The mean number of somatic embryos formed per explant was recorded after 4 and 8 weeks culture. Our findings indicated that the somatic embryogenesis in potato internodal segments could be evoked by an auxin (2,4-D) pulse treatment over a wide concentration and duration range. The results further suggested that a simple 20 microM 2,4-D pulse treatment could replace a lengthy 2 week induction phase in potato somatic embryogenesis and thus improve the system's practicability for wider uptake.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2,4-Dichlorophenoxyacetic Acid / pharmacology
  • Indoleacetic Acids / pharmacology*
  • Solanum tuberosum / cytology
  • Solanum tuberosum / drug effects
  • Solanum tuberosum / embryology*
  • Time Factors
  • Tissue Culture Techniques / methods*

Substances

  • Indoleacetic Acids
  • 2,4-Dichlorophenoxyacetic Acid