The impact of post-thaw colony-forming units-granulocyte/macrophage on engraftment following unrelated cord blood transplantation in pediatric recipients

Bone Marrow Transplant. 2007 May;39(9):515-21. doi: 10.1038/sj.bmt.1705629. Epub 2007 Mar 5.


We retrospectively reviewed the engraftment kinetics following unrelated cord blood transplantation (CBT) in association with the post-thaw colony-forming units-granulocyte/macrophage (CFU-GM) number along with the numbers of total nucleated cells (TNC), CD34(+) cells and CD3(+) cells. A total of 71 cord blood units prepared for 53 patients (double-unit CBT in 18 patients) were evaluated. Either the number of infused CFU-GM or CD34(+) cells was significantly lower in patients who failed to achieve engraftment (P=0.028 and 0.005, respectively). Post-thaw CFU-GM, TNC and CD34(+) cells correlated with the speed of neutrophil engraftment (P=0.004, 0.037 and 0.004, respectively), whereas only CFU-GM showed a significant correlation with platelet engraftment (r=-0.385, P=0.024). In double-unit transplants, the number of CFU-GM was the only significant factor predicting engraftment of the predominating unit (P=0.006). We conclude that the post-thaw CFU-GM number is a reliable predictor of rapid engraftment after CBT as well as of the predominating unit in double-unit transplants. Thus, it would be important to perform post-thaw CFU-GM assay on cryopreserved aliquots from several candidate cord blood units in advance before CBT to avoid selecting the unit that might possess a low clonogenic potential.

Publication types

  • Clinical Trial

MeSH terms

  • Adolescent
  • Antigens, CD34
  • Child
  • Child, Preschool
  • Colony-Forming Units Assay*
  • Cord Blood Stem Cell Transplantation*
  • Cryopreservation*
  • Graft Survival*
  • Granulocyte Precursor Cells*
  • Humans
  • Infant
  • Male
  • Predictive Value of Tests
  • Retrospective Studies
  • Transplantation, Homologous


  • Antigens, CD34