Dynamic changes in CREB phosphorylation and neuroadaptive gene expression in area V1 of adult monkeys after monocular enucleation

Mol Cell Neurosci. 2007 May;35(1):24-37. doi: 10.1016/j.mcn.2007.01.015. Epub 2007 Feb 6.


Our understanding of the molecular events that emerge after change in sensory input remains elusive, especially with regard to mature area V1. Here, we characterized P-CREB expression in area V1 of monkeys at multiple time-points after monocular enucleation (ME) to assess the possible contribution of CREB in visually deprived neocortex. Immunoblot assays and immunostainings showed that P-CREB is dynamically regulated in adult area V1, reaching a peak level between 5 and 30 days after ME, and becoming reduced at the 90-day post-ME time-point. This striking temporal increase in P-CREB level was paralleled by a concomitant increase of two CREB-regulated pro-survival effectors, namely Bcl-2 and Bcl-w. We present our results in the context of recent advances about adult visual neocortex and propose that ME induces a multifaceted CREB-mediated response that favors intrinsic stability of neurons and facilitates mature cortical networks to reorganize over a prolonged period.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological / physiology*
  • Animals
  • Apoptosis Regulatory Proteins / metabolism
  • Blindness / metabolism
  • Blindness / physiopathology*
  • Chlorocebus aethiops
  • Cyclic AMP Response Element-Binding Protein / genetics*
  • Cyclic AMP Response Element-Binding Protein / metabolism*
  • Eye Enucleation
  • Gene Expression
  • Immunoblotting
  • NF-kappa B / metabolism
  • Neuronal Plasticity / physiology
  • Phosphorylation
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • Visual Cortex / metabolism
  • Visual Cortex / physiology*


  • Apoptosis Regulatory Proteins
  • Cyclic AMP Response Element-Binding Protein
  • NF-kappa B
  • Proto-Oncogene Proteins c-bcl-2