Advances in citric acid fermentation by Aspergillus niger: biochemical aspects, membrane transport and modeling

Biotechnol Adv. May-Jun 2007;25(3):244-63. doi: 10.1016/j.biotechadv.2007.01.002. Epub 2007 Jan 19.


Citric acid is regarded as a metabolite of energy metabolism, of which the concentration will rise to appreciable amounts only under conditions of substantive metabolic imbalances. Citric acid fermentation conditions were established during the 1930s and 1940s, when the effects of various medium components were evaluated. The biochemical mechanism by which Aspergillus niger accumulates citric acid has continued to attract interest even though its commercial production by fermentation has been established for decades. Although extensive basic biochemical research has been carried out with A. niger, the understanding of the events relevant for citric acid accumulation is not completely understood. This review is focused on citric acid fermentation by A. niger. Emphasis is given to aspects of fermentation biochemistry, membrane transport in A. niger and modeling of the production process.

Publication types

  • Review

MeSH terms

  • Aspergillus niger / metabolism*
  • Biological Transport
  • Cell Membrane / metabolism*
  • Citric Acid / chemistry*
  • Citric Acid / metabolism*
  • Fermentation / physiology*
  • Models, Biological*


  • Citric Acid