Kaurane diterpenoids from Isodon excisus inhibit LPS-induced NF-kappaB activation and NO production in macrophage RAW264.7 cells

J Nat Prod. 2007 Apr;70(4):632-6. doi: 10.1021/np060638+. Epub 2007 Mar 6.


As part of an ongoing search for plant-derived compounds that inhibit the activation of NF-kappaB, the methanol extract of the aerial parts of Isodon excisus was found to have significant inhibitory effects on the activation of NF-kappaB in murine macrophage RAW264.7 cells. Bioactivity-guided isolation of the extract yielded five new diterpenoids, excisusin A-E (1-5), along with seven known compounds, inflexarabdonin I (6), inflexarabdonin G (7), inflexin (8), inflexanin A (9), inflexanin B (10), inflexinol (11), and inflexarabdonin A (12). The structures were determined by analysis of the spectroscopic data including 2D NMR. All of the isolates were evaluated for their inhibitory effects on LPS-induced NF-kappaB activation and nitric oxide production in RAW264.7 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Diterpenes, Kaurane* / chemistry
  • Diterpenes, Kaurane* / isolation & purification
  • Diterpenes, Kaurane* / pharmacology
  • Isodon / chemistry*
  • Korea
  • Lipopolysaccharides / pharmacology*
  • Macrophages / drug effects*
  • Mice
  • Molecular Conformation
  • Molecular Structure
  • NF-kappa B / antagonists & inhibitors*
  • Nitric Oxide / antagonists & inhibitors*
  • Plants, Medicinal / chemistry*


  • Diterpenes, Kaurane
  • Lipopolysaccharides
  • NF-kappa B
  • inflexanin B
  • Nitric Oxide