Control of DNA synthesis genes in fission yeast by the cell-cycle gene cdc10+

Nature. 1992 Jan 30;355(6359):449-53. doi: 10.1038/355449a0.

Abstract

In the budding yeast Saccharomyces cerevisiae, cell-cycle control over DNA synthesis occurs partly through the coordinate expression in late G1 phase of many, if not all, of the genes required for DNA synthesis. A cis-acting hexamer element ACGCGT (an MluI restriction site) is responsible for coordinating transcriptional regulation of these genes at the G1/S phase boundary and we have identified a binding activity, DSC1, that recognizes these sequences in a cell-cycle-dependent manner. In the distantly related fission yeast Schizosaccharomyces pombe, only one of the known DNA synthesis genes, cdc22+, which encodes a subunit of ribonucleotide reductase, is periodically expressed in late G1 (ref. 6). The promoter region of cdc22+ has two MluI sites and five related sequences, suggesting that similar controls over DNA synthesis genes could occur in fission yeast. We report here a binding activity in fission yeast that is very similar to DSC1 in budding yeast. We also show that the fission yeast cdc10+ gene product, which is required for Start and entry into S phase, is a component of this binding activity.

Publication types

  • Comparative Study

MeSH terms

  • Alleles
  • Base Sequence
  • Cell Cycle*
  • DNA Replication / genetics*
  • DNA, Fungal / genetics
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Genes, Fungal*
  • Molecular Sequence Data
  • Mutagenesis
  • Oligodeoxyribonucleotides
  • Plasmids
  • Polymerase Chain Reaction / methods
  • Promoter Regions, Genetic
  • Restriction Mapping
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / genetics*
  • Schizosaccharomyces / cytology
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / growth & development
  • Temperature

Substances

  • DNA, Fungal
  • Fungal Proteins
  • Oligodeoxyribonucleotides