Discrimination of C57BL/6J Rj and 129S2/SvPasCrl inbred mouse strains by use of simple sequence length polymorphisms

J Am Assoc Lab Anim Sci. 2007 Mar;46(2):21-4.

Abstract

We present a list of simple sequence length polymorphisms (SSLPs) that was used in a polymerase chain reaction (PCR)-based marker-assisted selection protocol to facilitate the generation of a C57BL/6J Rj congenic strain from a mixed 129S2/SvPasCrlxC57BL/6J Rj background. We chose informative SSLP markers that would permit evaluation of the PCR products on NuSieve agarose or nondenaturing polyacrylamide gels. This list of oligonucleotide pairs is useful for analyzing and backcrossing knockout or transgenic strains derived from 129S2/SvPasCrl or C57BL/6J Rj backgrounds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Primers
  • Mice
  • Mice, Congenic / genetics*
  • Mice, Inbred C57BL / genetics*
  • Mice, Inbred Strains / classification*
  • Mice, Inbred Strains / genetics
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*

Substances

  • DNA Primers