The effects of polyphenols in olive leaves on platelet function

Nutr Metab Cardiovasc Dis. 2008 Feb;18(2):127-32. doi: 10.1016/j.numecd.2006.09.001. Epub 2007 Mar 7.


Introduction: The phenolic compounds of olive leaves and olive oils in the Mediterranean diet have been associated with a reduced incidence of heart disease. Accordingly, antioxidant-rich diets may prevent the deleterious effects of oxidative metabolism by scavenging free radicals, thus inhibiting oxidation and delaying atherosclerosis. The process involves phospholipase C activation and arachidonic acid metabolism, and is thought to reduce hydrogen peroxide (H(2)O(2)). In our study, an extract of Olea europaea L. leaves was used. The active phenolic compounds in this extract are part of the secoiridoid family, known for their capacity to scavenge H(2)O(2). The results from this study will help to improve our understanding of effects of polyphenol antioxidants in olive leaf extract on platelet function.

Methods: Full blood examination (FBE), platelet aggregation, and ATP release were performed on samples from fasting, normal, healthy male subjects. Platelet function at increasing concentrations of oleuropein was investigated through measures of platelet aggregation and ATP release from activated platelets.

Results: Blood analysis (n=11) revealed a significant dose-dependant reduction in platelet activity with olive extract concentrations of 1.0% v/v (P<0.001). ATP Release showed a similar pattern (P=0.02).

Conclusions: Olive leaf polyphenols derived from O. europaea L. leaves inhibited in vitro platelet activation in healthy, non-smoking males. Further bioavailability studies need to be undertaken to determine the in vivo effect of extract on platelet function and to validate the present results.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Adult
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology*
  • Blood Platelets / drug effects*
  • Blood Platelets / metabolism
  • Dose-Response Relationship, Drug
  • Flavonoids / isolation & purification
  • Flavonoids / pharmacology*
  • Humans
  • In Vitro Techniques
  • Iridoid Glucosides
  • Iridoids
  • Male
  • Middle Aged
  • Olea* / chemistry
  • Phenols / isolation & purification
  • Phenols / pharmacology*
  • Plant Extracts / pharmacology
  • Plant Leaves
  • Platelet Aggregation / drug effects*
  • Polyphenols
  • Pyrans / isolation & purification
  • Pyrans / pharmacology*


  • Antioxidants
  • Flavonoids
  • Iridoid Glucosides
  • Iridoids
  • Phenols
  • Plant Extracts
  • Polyphenols
  • Pyrans
  • oleuropein
  • Adenosine Triphosphate