One-step transformation of yeast in stationary phase

Curr Genet. 1992 Jan;21(1):83-4. doi: 10.1007/BF00318659.

Abstract

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45 degrees C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5 x 10(8) cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 10(4) transformants/microgram plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

MeSH terms

  • DNA, Fungal / genetics*
  • Schizosaccharomyces / genetics
  • Transformation, Genetic*
  • Yeasts / genetics*
  • Yeasts / growth & development

Substances

  • DNA, Fungal