Creating recombination-activated genes and sequence-defined mutant libraries using transposons

Methods Enzymol. 2007:421:126-40. doi: 10.1016/S0076-6879(06)21012-7.

Abstract

The properties of a collection of transposon Tn5 derivatives that generate reporter gene fusions and internal protein tags are summarized. Procedures utilizing several of the transposons for generating genes activated by Cre-loxP recombination and for creating large sequence-defined mutant libraries are described in detail.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Base Sequence
  • DNA Transposable Elements*
  • Gene Library*
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Mutation*
  • Recombination, Genetic*

Substances

  • DNA Transposable Elements