Implants of type I collagen gel containing MG-63 osteoblast-like cells can act as stable scaffolds stimulating the bone healing process at the sites of the surgically-produced segmental diaphyseal defects in male rabbits

In Vivo. 2007 Jan-Feb;21(1):69-76.


Background: Three-dimensional (3-D) type I collagen gel culture systems allow long-term growth of osteoblast-like cells, in vitro. Whether the implantation of 3-D collagen systems can stimulate new bone formation was assessed in male rabbits.

Materials and methods: A 10-mm segmental diaphyseal defect was surgically produced at the left and right limbs of 50 adult male rabbits. The 3-D systems containing MG-63 osteoblast-like cells were implanted at the right-limb defects of all 50 animals. Twenty-five left-limb defects were implanted with 3-D collagen gels containing no MG-63 cells, while the rest were left empty. The bone repair process was serially assessed by radiography for up to 8 weeks and by histological analysis for up to the week 32 post-surgery.

Results: Ninety-four per cent (94%) of the right-limb defects, presented radiographic evidence of complete bone-end bridging within 8 weeks. None of the 50 left-limb defects presented radiographic post-implantation evidence of bone-end bridging. The radiographic evidence of the bone-end bridging was corroborated with histological evidence of new bone formation, while the medullar canals were filled with bone marrow elements.

Conclusion: Implants of the 3-D collagen gels containing osteoblast-like cells can be used as stable scaffolds allowing the migration/proliferation of the bone regenerating cells in male rabbits.

MeSH terms

  • Animals
  • Bone Diseases / diagnostic imaging
  • Bone Diseases / physiopathology
  • Bone Diseases / therapy*
  • Bone and Bones / diagnostic imaging
  • Bone and Bones / physiopathology*
  • Cell Line
  • Collagen Type I / therapeutic use*
  • Diaphyses / diagnostic imaging
  • Diaphyses / physiopathology
  • Gels
  • Humans
  • Male
  • Osteoblasts / transplantation*
  • Osteogenesis
  • Prostheses and Implants*
  • Rabbits
  • Radiography
  • Upper Extremity / diagnostic imaging
  • Upper Extremity / physiopathology
  • Wound Healing*


  • Collagen Type I
  • Gels