Clinical significance of ELISA positive and immunofluorescence negative anti-dsDNA antibody

Clin Chim Acta. 2007 May 1;380(1-2):182-5. doi: 10.1016/j.cca.2007.02.010. Epub 2007 Feb 15.


Background: Positivity for anti-dsDNA antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). In the present study, the significance of ELISA positive and Crithidia luciliae immunofluorescence test (CLIFT) negative anti-dsDNA sera was evaluated.

Methods: There were 371 consecutive serum samples submitted to for anti-dsDNA testing that were assayed using anti-dsDNA ELISA and CLIFT. Sera showing discrepant results were collected and then examined using 3 commercial anti-dsDNA and anti-ssDNA ELISA kits and by Farr assay. Medical records were reviewed for those patients who were ELISA positive and CLIFT negative for anti-dsDNA.

Results: Fifty-two patients of 100 anti-dsDNA ELISA positive patients were negative by CLIFT. For ELISA positive and CLIFT negative sera, Farr assays showed the highest positive rate (72.7%) for the 4 different anti-dsDNA assays (3 commercial kits and the Farr assay). Nearly 80% of 44 ELISA positive and CLIFT negative patients met >or=3 of the SLE classification criteria (excluding the anti-dsDNA criterion).

Conclusion: Some anti-dsDNA ELISA kits have diagnostic efficiencies that are similar to that of the Farr assay. Moreover, the study identifies a group of patients that are ELISA positive but CLIFT negative for anti-dsDNA, and indicates that the majority of these patients have clinically relevant SLE.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Antibodies, Antinuclear / blood*
  • Autoantibodies / blood*
  • Crithidia / immunology
  • DNA / immunology*
  • Enzyme-Linked Immunosorbent Assay*
  • Female
  • Fluorescent Antibody Technique, Indirect*
  • Humans
  • Lupus Erythematosus, Systemic / diagnosis
  • Lupus Erythematosus, Systemic / immunology*
  • Male
  • Predictive Value of Tests
  • Radioimmunoprecipitation Assay
  • Recombinant Proteins / immunology


  • Antibodies, Antinuclear
  • Autoantibodies
  • Recombinant Proteins
  • DNA