Dysregulation of placental endothelial lipase and lipoprotein lipase in intrauterine growth-restricted pregnancies

J Clin Endocrinol Metab. 2007 Jun;92(6):2256-63. doi: 10.1210/jc.2006-2403. Epub 2007 Mar 13.


Context: Fetal supply of maternally derived fatty acids requires lipase-mediated hydrolysis of lipoprotein-borne triglycerides and phospholipids at the placental surface.

Objective: The objective of the study was to test the hypothesis that members of the triglyceride lipase gene (TLG) family are expressed in the human placenta at the maternoplacental (syncytiotrophoblast) and fetoplacental (endothelial cells) interface and that their expression is altered in pregnancy pathologies.

Design and setting: Expression of TLG family members in primary placental cells (trophoblast and endothelial cells) and tissues of first-trimester and term human placenta was analyzed by microarrays, RT-PCR, Western blotting, and immunohistochemistry. Their expression was compared between normal pregnancies and those complicated with intrauterine growth restriction (IUGR).

Participants: Participants included women with uncomplicated pregnancies and pregnancies complicated by IUGR.

Results: Endothelial lipase (EL) and lipoprotein lipase (LPL) were the only lipases among the TLG family expressed in key cells of the human placenta. In first trimester, EL and LPL were expressed in trophoblasts. At term, EL was detected in trophoblasts and endothelial cells, whereas LPL was absent in these cells. Both lipases were found at placental blood vessels, EL in vascular endothelial cells and LPL in the surrounding smooth muscle cells. In total placental tissue EL expression prevails in first trimester and at term. Compared with normal placentas, EL mRNA was decreased (30%; P < 0.02), whereas LPL mRNA expression was increased (2.4-fold; P < 0.015) in IUGR.

Conclusion: EL is the predominant TLG family member in the human placenta present at both interfaces. EL and LPL are dysregulated in IUGR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Blotting, Western
  • Cells, Cultured
  • Endothelial Cells / cytology
  • Endothelial Cells / enzymology*
  • Family Health
  • Female
  • Fetal Growth Retardation / genetics
  • Fetal Growth Retardation / pathology
  • Fetal Growth Retardation / physiopathology*
  • Gene Expression Regulation, Enzymologic
  • Humans
  • Immunohistochemistry
  • Lipase / genetics*
  • Lipase / metabolism
  • Lipoprotein Lipase / genetics*
  • Lipoprotein Lipase / metabolism
  • Maternal-Fetal Exchange
  • Oligonucleotide Array Sequence Analysis
  • Pregnancy
  • Trophoblasts / cytology
  • Trophoblasts / enzymology*


  • LIPG protein, human
  • Lipase
  • Lipoprotein Lipase