Noninfectious entry of HIV-1 into peripheral and brain macrophages mediated by the mannose receptor

Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5097-102. doi: 10.1073/pnas.0611263104. Epub 2007 Mar 14.


Although protein receptors on the plasma membrane involved in the initial steps of productive HIV-1 infection have been well characterized, little is known about interactions between cellular carbohydrate receptors and HIV-1. Here, we report the involvement of a carbohydrate receptor, the macrophage mannose receptor (MR), and its role in supporting HIV-1 binding and entry. HIV-1 can enter the cytoplasm of human macrophages and microglia as well as murine macrophages by MR, although no subsequent viral replication was observed. Correspondingly, HIV-1 entry into Cos-7 cells after induction of expression of MR by transfection with MR-cDNA did not demonstrate viral replication. Our studies suggest that whereas MR may serve as a binding and an entry site, the MR-mediated pathway does not lead to productive HIV-1 infection. In addition, we report that recombinant HIV-1 gp120 blocks MR-mediated phagocytosis in human and murine alveolar macrophages and microglial cells. Therefore, characterization of the HIV-1 noninfectious MR-mediated phagocytic pathway may foster advances in HIV-1 vaccine design and an improved understanding of HIV-1/AIDS pathogenesis and host defenses.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / cytology*
  • Brain / virology*
  • COS Cells
  • Chlorocebus aethiops
  • DNA, Complementary / metabolism
  • HIV Envelope Protein gp120
  • HIV-1 / pathogenicity
  • HIV-1 / physiology*
  • Humans
  • Lectins, C-Type / metabolism*
  • Macrophages, Alveolar / cytology
  • Macrophages, Alveolar / microbiology
  • Macrophages, Alveolar / virology*
  • Mannose Receptor
  • Mannose-Binding Lectins / metabolism*
  • Mice
  • Microglia / cytology
  • Microglia / microbiology
  • Microglia / virology
  • Models, Biological
  • Phagocytosis
  • Pneumocystis carinii
  • Protein Binding
  • Receptors, Cell Surface / metabolism*
  • Recombinant Proteins / metabolism
  • Transfection
  • Virus Internalization*


  • DNA, Complementary
  • HIV Envelope Protein gp120
  • Lectins, C-Type
  • Mannose Receptor
  • Mannose-Binding Lectins
  • Receptors, Cell Surface
  • Recombinant Proteins