Assessment of oxidative stress due to exposure to pesticides in plasma and urine of traditional limited-resource farm workers: formation of the DNA-adduct 8-hydroxy-2-deoxy-guanosine (8-OHdG)

J Environ Sci Health B. 2007 Feb;42(2):151-5. doi: 10.1080/03601230601123276.


The objective of this study was to assess the risk of genotoxicity caused due to oxidative stress using plasma and urinary levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) in farm workers for six months during a growing season. Blood and urine samples were collected once a month for six months (June to November 2003) from farm workers (n = 15) and urban unexposed controls (n = 10). Plasma and urinary levels of 8-OHdG were evaluated by Enzyme Linked Immunosorbent Assay (ELISA) technique. There was no significant difference in the urinary levels of 8-OHdG between the farm workers and the control group, but there was an approximately four-fold increase in mean values of plasma 8-OHdG levels in the farm worker group (P < or = 0.05).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Biomarkers / blood
  • Biomarkers / urine
  • DNA Adducts
  • DNA Damage
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / blood
  • Deoxyguanosine / urine
  • Environmental Monitoring
  • Female
  • Humans
  • Male
  • Middle Aged
  • Occupational Exposure*
  • Oxidative Stress* / drug effects
  • Pesticides / adverse effects*
  • Risk Factors
  • Seasons
  • Transients and Migrants


  • Biomarkers
  • DNA Adducts
  • Pesticides
  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine