Abstract
The Tax trans-activator protein of the type I human T-cell leukemia virus is expressed predominantly in the nuclei of cells. However, this viral trans-activator is distinguished from most other nuclear proteins by the absence of a short highly basic nuclear localization signal. Previous mutational analyses of the tax gene revealed that many of the missense mutations involving the amino terminus of Tax resulted in a predominantly cytoplasmic pattern of expression. We now report that the amino terminal 48 residues of Tax comprise a functional nuclear localization signal as demonstrated by the ability of this region to retarget expression of a large cytoplasmic protein to the nucleus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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DNA Mutational Analysis
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Gene Products, tax / chemistry*
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Gene Products, tax / genetics
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Gene Products, tax / metabolism
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Human T-lymphotropic virus 1 / chemistry*
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Human T-lymphotropic virus 1 / genetics
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Human T-lymphotropic virus 1 / metabolism
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Microscopy, Fluorescence
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Molecular Sequence Data
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Nuclear Localization Signals
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Nuclear Proteins / chemistry*
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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Gene Products, tax
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Nuclear Localization Signals
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Nuclear Proteins
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Recombinant Fusion Proteins
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beta-Galactosidase