Nonreplicating viral vectors as potential vaccines: recombinant canarypox virus expressing measles virus fusion (F) and hemagglutinin (HA) glycoproteins

Virology. 1992 Mar;187(1):321-8. doi: 10.1016/0042-6822(92)90321-f.

Abstract

The development of canarypox virus (CPV) recombinants expressing the hemagglutinin (HA) and fusion (F) glycoproteins of measles virus (MV) is described. Inoculation of the CPV-MV recombinants into avian or nonavian tissue culture substrates led to the expression of authentic MVF and MVHA as determined by radioimmunoprecipitation and surface immunofluorescence. In contrast to avian-derived tissue culture, no productive replication of the CPV recombinant was evident in tissue culture cells derived from nonavian origin. On inoculation of dogs, a species restricted for avipoxvirus replication, the recombinants elicited a protective immune response against a lethal canine distemper virus (CDV) challenge. The level of MV neutralizing antibodies and the level of protection induced against CDV challenge achieved by the host-restricted CPV vector were equivalent to that obtained by vaccinia virus vectors expressing the same MV antigens.

MeSH terms

  • Animals
  • Antibodies, Viral / biosynthesis*
  • Cells, Cultured
  • Defective Viruses / genetics
  • Defective Viruses / immunology
  • Distemper / prevention & control
  • Distemper Virus, Canine / immunology
  • Dogs
  • Genetic Vectors / genetics
  • Genetic Vectors / immunology
  • Hemagglutinins, Viral / genetics
  • Hemagglutinins, Viral / immunology*
  • Measles virus / genetics
  • Measles virus / immunology*
  • Microscopy, Fluorescence
  • Plasmids / genetics
  • Poxviridae / genetics*
  • Poxviridae / immunology
  • Precipitin Tests
  • Vaccines, Synthetic / immunology
  • Vaccinia virus / genetics
  • Vaccinia virus / immunology
  • Viral Fusion Proteins / genetics
  • Viral Fusion Proteins / immunology*
  • Viral Vaccines / immunology*

Substances

  • Antibodies, Viral
  • Hemagglutinins, Viral
  • Vaccines, Synthetic
  • Viral Fusion Proteins
  • Viral Vaccines