[Inhibition of EBV-encoded LMP-1 by DNA-based RNA interference affects metastatic ability of nasopharyngeal carcinoma cells]

Zhonghua Zhong Liu Za Zhi. 2006 Oct;28(10):724-7.
[Article in Chinese]


Objective: To evaluate the application of DNA vector-based RNA interference for silencing LMP-1 expression in EBV-positive nasopharyngeal cancer (NPC) C611 cells and analyze the inhibiting effects of LMP-1 silencing on metastatic property of NPC cells.

Methods: DNA vectors with LMP-1 shRNA template were transfected into C611 cells. After G418 selection, a LMP-1 silenced sub-clone named C611L was isolated. Effect of LMP-1 inhibition on its metastatic properties was evaluated by cell adhesion, invasion and wound healing assays.

Results: shRNA vector mediated stable RNAi against LMP-1 in C611 cells. Loss of LMP-1 significantly altered cell motility, substratum adhesion and transmembrane invasion ability.

Conclusion: Stable suppression of LMP-1 induced by shRNA vector can inhibit metastatic properties of NPC cells.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cadherins / metabolism
  • Cell Adhesion
  • Cell Line, Tumor
  • Cell Movement
  • Genetic Vectors
  • Herpesvirus 4, Human / genetics*
  • Herpesvirus 4, Human / growth & development
  • Humans
  • Nasopharyngeal Neoplasms / metabolism
  • Nasopharyngeal Neoplasms / pathology
  • Nasopharyngeal Neoplasms / virology
  • Neoplasm Metastasis
  • RNA Interference*
  • RNA, Small Interfering / genetics*
  • Transfection
  • Viral Matrix Proteins / genetics*
  • Viral Matrix Proteins / physiology


  • Cadherins
  • EBV-associated membrane antigen, Epstein-Barr virus
  • RNA, Small Interfering
  • Viral Matrix Proteins