Characterization of PsTX-60B, a new membrane-attack complex/perforin (MACPF) family toxin, from the venomous sea anemone Phyllodiscus semoni

Hiroshi Satoh; Naomasa Oshiro; Setsuko Iwanaga; Michio Namikoshi; Hiroshi Nagai

doi:10.1016/j.toxicon.2007.01.006

Characterization of PsTX-60B, a new membrane-attack complex/perforin (MACPF) family toxin, from the venomous sea anemone Phyllodiscus semoni

Toxicon. 2007 Jun 15;49(8):1208-10. doi: 10.1016/j.toxicon.2007.01.006. Epub 2007 Jan 25.

Authors

Hiroshi Satoh¹, Naomasa Oshiro, Setsuko Iwanaga, Michio Namikoshi, Hiroshi Nagai

Affiliation

¹ Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.

PMID: 17368498
DOI: 10.1016/j.toxicon.2007.01.006

Abstract

The sea anemone Phyllodiscus semoni has been known as one of the most venomous sea anemones. Previously, we reported the isolation of the major lethal protein toxins, PsTX-60A and PsTX-60B, from P. semoni and the primary structure of PsTX-60A. In this paper, we report the complete sequence of cDNA (1600 base pairs) encoding PsTX-60B and the deduced primary structure (488 amino acids) of PsTX-60B. The amino acid sequence of PsTX-60B showed the homology with those of PsTX-60A and Actineria villosa toxin, AvTX-60A. The results showed that PsTX-60B is a new member of the membrane-attack complex/perforin (MACPF) family toxin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cnidarian Venoms / genetics*
DNA, Complementary / genetics
Molecular Sequence Data
Perforin / genetics*
Sea Anemones / chemistry*
Sequence Analysis, DNA
Sequence Homology

Substances

Cnidarian Venoms
DNA, Complementary
Perforin