Establishment of a human herpes virus-8-negative malignant effusion lymphoma cell line (STR-428) carrying concurrent translocations of BCL2 and c-MYC genes

Leuk Res. 2007 Sep;31(9):1285-92. doi: 10.1016/j.leukres.2006.12.023. Epub 2007 Mar 21.

Abstract

A new cell line, STR-428 was established from ascites tumor cells of a malignant effusion lymphoma patient without human herpes virus-8 (HHV-8) infection. STR-428 cells showed an immunophenotype of mature B-cells and produced few cytokines related to lymphomatous effusion. Karyotypic and genetic analysis revealed complex translocations including t(14;18)(q32;q21) effecting IgH/BCL2 and der(8)t(3;8)(q27;q24) involving c-MYC. STR-428 represents a unique, B-cell lymphoma cell line carrying concurrent rearrangement of BCL2 and c-MYC genes with features distinct from those of HHV-8-related primary effusion lymphoma. This cell line may be a valuable tool, other than HHV-8, to investigate the pathogenesis of primary lymphomatous effusion.

MeSH terms

  • Blotting, Southern
  • Chromosomes, Human, Pair 14 / genetics
  • Chromosomes, Human, Pair 18 / genetics
  • Cytokines
  • Herpesvirus 8, Human / physiology*
  • Humans
  • Immunoenzyme Techniques
  • Immunophenotyping
  • In Situ Hybridization, Fluorescence
  • Lymphoma / genetics*
  • Lymphoma / metabolism
  • Lymphoma / virology
  • Male
  • Middle Aged
  • Pleural Effusion, Malignant / genetics*
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins c-bcl-2 / genetics*
  • Proto-Oncogene Proteins c-myc / genetics*
  • Translocation, Genetic*
  • Tumor Cells, Cultured

Substances

  • Cytokines
  • MYC protein, human
  • Proto-Oncogene Proteins c-bcl-2
  • Proto-Oncogene Proteins c-myc