The Use of a Combined Enrichment-Filtration Technique for the Isolation of Campylobacter Spp. From Clinical Samples

Clin Microbiol Infect. 2007 Jun;13(6):643-4. doi: 10.1111/j.1469-0691.2007.01712.x. Epub 2007 Mar 19.

Abstract

A combined non-selective enrichment-filtration technique was investigated for the isolation of Campylobacter spp. from clinical samples. In total, 479 samples were tested by direct culture, enrichment subculture and enrichment-filtration. The enrichment-filtration technique was used with both selective and non-selective media. Direct culture and enrichment subculture yielded 13 and seven isolates, respectively, while enrichment-filtration yielded 18 and 14 isolates on selective and non-selective agar, respectively. Thus, the combination of enrichment-filtration with selective agar produced a 38.5% increase in the number of isolates (p <0.05). All isolates were identified as Campylobacter jejuni.

MeSH terms

  • Bacteriological Techniques / methods*
  • Bodily Secretions / microbiology
  • Campylobacter / growth & development
  • Campylobacter / isolation & purification*
  • Campylobacter Infections / microbiology*
  • Culture Media
  • Filtration / methods*
  • Humans

Substances

  • Culture Media