Effect of transforming growth factor beta and basic fibroblast growth factor on steroid-impaired healing intestinal wounds

Br J Surg. 1992 Jan;79(1):69-72. doi: 10.1002/bjs.1800790124.


A longitudinal intestinal wound model in the pig was used to assess the effect of parenteral steroids (betamethasone 12 mg 50 kg-1 intramuscularly twice daily) on breaking load. Steroid treatment significantly decreased the breaking load of wounds in the ileum and colon in comparison with wounds from saline-treated animals. In a further group of animals receiving steroids, paired longitudinal wounds were constructed. One wound of a pair was treated with a local application of transforming growth factor beta (TGF-beta) (5 micrograms per wound) or basic fibroblast growth factor (5 micrograms per wound) in a collagen suspension. The other wound was treated with a collagen suspension alone. Ileal wounds treated with TGF-beta were significantly stronger than collagen-treated controls at 7 days. The steroid-induced impairment of breaking load in intestinal wounds is partially reversed by a local application of TGF-beta in a collagen suspension at the time of surgery.

MeSH terms

  • Animals
  • Betamethasone / antagonists & inhibitors
  • Fibroblast Growth Factor 2 / pharmacology*
  • Intestines / physiology
  • Intestines / surgery*
  • Stress, Mechanical
  • Swine
  • Tensile Strength
  • Transforming Growth Factor beta / pharmacology*
  • Wound Healing / drug effects*


  • Transforming Growth Factor beta
  • Fibroblast Growth Factor 2
  • Betamethasone