Human clonal CD8 autoreactivity to an IGRP islet epitope shared between mice and men

Ann N Y Acad Sci. 2007 Apr;1103:192-5. doi: 10.1196/annals.1394.024. Epub 2007 Mar 21.

Abstract

Type 1 diabetes (T1D) is a multifactorial disease characterized by the infiltration and subsequent destruction of the pancreatic insulin-producing beta cells by autoreactive T cells. CD8(+) T cells play an essential role in this beta cell destruction. However, little is known about the target antigens of CD8(+) T cells in human T1D patients. The aim of this study was to assess whether an epitope derived from the islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP), IGRP(265-273,) which has recently been identified as a target in non-obese diabetic (NOD) mice and is fully homologous to the human epitope, is a target of human diabetogenic CD8(+) T cells. We isolated a human CD8 T cell clone against this epitope, which confirms that this IGRP epitope is shared across species.

MeSH terms

  • Animals
  • Autoantigens / immunology*
  • CD8 Antigens / immunology*
  • Diabetes Mellitus, Type 1 / immunology*
  • Disease Models, Animal
  • Epitopes / immunology
  • Glucose-6-Phosphatase / immunology*
  • Humans
  • Islets of Langerhans / immunology*
  • Mice
  • Proteins / immunology*

Substances

  • Autoantigens
  • CD8 Antigens
  • Epitopes
  • Proteins
  • Glucose-6-Phosphatase
  • G6PC2 protein, human
  • G6pc2 protein, mouse