Uptake of Minocycline by Escherichia Coli

J Antimicrob Chemother. 1992 Jan;29(1):19-25. doi: 10.1093/jac/29.1.19.


Uptake of tetracyclines into Escherichia coli was assessed with a strain carrying a tetA-lacZ translational fusion, in which expression of the enzyme is controlled by the pSC101 tetR repressor gene, by examining beta-galactosidase induction. The ability of tetracycline analogues to induce beta-galactosidase synthesis was correlated with their hydrophobicity, such that hydrophobic analogues were poor enzyme inducers. Treatment of E. coli with polymyxin B nonapeptide (PMBN) rendered cells more permeable to minocycline, but not to tetracycline.

MeSH terms

  • Enzyme Induction
  • Escherichia coli / metabolism*
  • Minocycline / pharmacokinetics*
  • Polymyxin B / analogs & derivatives
  • Polymyxin B / pharmacology
  • Tetracyclines / pharmacokinetics
  • beta-Galactosidase / biosynthesis


  • Tetracyclines
  • polymyxin B nonapeptide
  • beta-Galactosidase
  • Minocycline
  • Polymyxin B