A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C

Microbiology (Reading). 2007 Apr;153(Pt 4):1198-1206. doi: 10.1099/mic.0.2006/002287-0.


An unknown cytotoxin was identified in the culture supernatant of Clostridium perfringens type C. The cytotoxin, named TpeL, which was purified using mAb-based affinity chromatography, had a lethal activity of 62 minimum lethal dose (MLD) mg(-1) in mice and a cytotoxic activity of 6.2x10(5) cytotoxic units (CU) mg(-1) in Vero cells. The nucleotide sequence of TpeL was determined. The entire ORF had a length of 4953 bases, and the same nucleotide sequence was not recorded in the GenBank/EMBL/DDBJ databases. The molecular mass calculated from the deduced amino acid sequence was 191 kDa, and a signal peptide region was not found within the ORF. The deduced amino acid sequence exhibited 30-39 % homology to Clostridium difficile toxins A (TcdA) and B (TcdB), Clostridium sordellii lethal toxin (TcsL) and Clostridium novyi alpha-toxin (TcnA). The amino acid sequence of TpeL is shorter than these toxins, and the homologous region was located at the N-terminal site. Eighteen strains of C. perfringens types A, B and C were surveyed for the presence of the tpeL gene by PCR. The tpeL gene was detected in all type B (one strain) and C strains (five strains), but not in any type A strains (12 strains). TpeL was detected in culture filtrates of the five type C strains by dot-blot analysis, but not in the type B strain. It was concluded that TpeL is a novel toxin similar to the known large clostridial cytotoxins. Furthermore, the data indicated that TpeL is produced by many C. perfringens type C strains.

MeSH terms

  • Animals
  • Bacterial Toxins / chemistry
  • Bacterial Toxins / genetics
  • Bacterial Toxins / isolation & purification*
  • Bacterial Toxins / toxicity
  • Cattle
  • Chlorocebus aethiops
  • Clostridium perfringens / chemistry*
  • Clostridium perfringens / genetics
  • Clostridium perfringens / isolation & purification
  • Cytotoxins / chemistry
  • Cytotoxins / genetics
  • Cytotoxins / isolation & purification
  • Cytotoxins / toxicity
  • Mice
  • Molecular Sequence Data
  • Phylogeny
  • Swine
  • Vero Cells


  • Bacterial Toxins
  • Cytotoxins

Associated data

  • GENBANK/AB262081