Abstract
MicroRNA (miRNA)-mediated gene silencing is one of the major regulatory pathways in eukaryotes. Much effort has been made to identify the factors involved in the pathway, and our understanding of RNA silencing has significantly advanced in recent years. Our group has been working on some of the issues regarding miRNA biogenesis and, in this paper, we summarize what we and other workers in the field have learned thus far. The focus remains on the role of Drosha and DGCR8 in the early events of miRNA biogenesis in animals.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Caenorhabditis elegans Proteins / chemistry
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism
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Drosophila Proteins / chemistry
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Drosophila Proteins / genetics
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Drosophila Proteins / metabolism
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Gene Silencing
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Humans
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MicroRNAs / genetics*
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MicroRNAs / metabolism*
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Models, Biological
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Multiprotein Complexes
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Protein Structure, Tertiary
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Proteins / chemistry
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Proteins / genetics
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Proteins / metabolism
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RNA Processing, Post-Transcriptional
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RNA-Binding Proteins
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Ribonuclease III / chemistry
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Ribonuclease III / genetics
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Ribonuclease III / metabolism*
Substances
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Caenorhabditis elegans Proteins
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DGCR8 protein, human
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Drosophila Proteins
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MicroRNAs
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Multiprotein Complexes
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Proteins
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RNA-Binding Proteins
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DROSHA protein, human
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Ribonuclease III
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drosha protein, Drosophila
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drsh-1 protein, C elegans